Anti-PRKAG3 antibody [A7-C7]

Anti-PRKAG3 antibody [A7-C7]

• 概述

• 产品描述AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enz

• 产品名称Anti-PRKAG3 antibody [A7-C7]

• 分子量54 kDa

• 种属反应性Human

• 验证应用WB,ICC,IHC-P

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Cytosol, extracellular space, nucleoplasm, nucleotide-activated protein kinase complex.

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• ​其它名称

  more

  • 5 AMP activated protein kinase subunit gamma 3 antibody

  • 5'-AMP-activated protein kinase subunit gamma-3 antibody

  • AAKG3_HUMAN antibody

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

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  Fig1: Western blot analysis of PRKAG3 on human PRKAG3 recombinant protein using anti-PRKAG3 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PRKAG3 on HEK293 (1) and PRKAG3-hIgGFc transfected HEK293 (2) cell lysate using anti-PRKAG3 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining PRKAG3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human rectum tissue using anti-PRKAG3 antibody. Counter stained with hematoxylin.

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