Anti-AOF1 antibody

Anti-AOF1 antibody

• 概述

• 产品描述AOF1 (amine-oxidase flavin-containing domain 1), whose alternative names include KDM1B (lysine (K)-specific demethylase 1B) or LSD2 (lysine-specific histone demethylase 2), is an 823 amino acid nuclear protein belonging to the flavin monoamine oxidase family. As a histone demethylase, AOF1 specifically demethylates Lys4 of histone H3, a marker for epigenetic transcriptional activation, by interacting with a long stretch of the H3 N-terminal tail of mono- and dimethylated Lys4. Existing as three alternatively spliced isoforms, AOF1 is required for de novo DNA methylation of some imprinted genes in oocytes and contains a single SWIRM domain that is implicated in chromatin regulation. AOF1 contains one CW-type zinc finger and is encoded by a gene located on human chromosome 6p22.3.
• 产品名称Anti-AOF1 antibody
• 分子量92 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • amine oxidase (flavin containing) domain 1 antibody
  • amine oxidase, flavin containing 1 antibody
  • AOF1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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