Human TGF-beta RII / TGFBR2 Protein, His Tag

Human TGF-beta RII / TGFBR2 Protein, His Tag

分子别名（Synonym）

TGFBR2,TGFR2,TbetaR-II,TGFβR2

表达区间及表达系统（Source）

Human TGF-beta RII, His Tag is expressed from human 293 cells . It contains AA Thr 23 - Asp 159.

Predicted N-terminus: Thr 23

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 17.4 kDa. The protein migrates as 25-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human TGF-beta RII, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

活性（Bioactivity）-ELISA

Serial dilutions of Human TGF-beta RII, His Tagwere added into Human Latent TGFB1, His TagBiotinylated Human TGF-beta RII, His,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.39287 μg/mL (QC tested).

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