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Mouse VEGF R1 / Flt-1 Protein, Mouse IgG2a Fc Tag, low endotoxin

Mouse VEGF R1 / Flt-1 Protein, Mouse IgG2a Fc Tag, low endotoxin
别名: FLT,VEGFR1,FLT1
储存条件: -20℃ 
Mouse VEGF R1 / Flt-1 Protein, Mouse IgG2a Fc Tag, low endotoxin
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-VE1-M5256-100ug 100ug 现货 3000.00 3000
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熔点:
密度:
储存条件: -20℃

 

Mouse VEGF R1 / Flt-1 Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

FLT,VEGFR1,FLT1

表达区间及表达系统(Source)

Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxinis expressed from human 293 cells. It contains AA Tyr 23 - Glu 759 .

Predicted N-terminus: Tyr 23

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 109.8 kDa. The protein migrates as 140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>85% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE1-M5256) SDS-PAGE gel

Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human VEGF165, Tag Free at 2 μg/mL (100 μL/well) can bind Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 2-31 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human VEGF-B, His Tagat 2 μg/mL (100 μL/well) can bind Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxin  with a linear range of 8-63 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Mouse VEGF120, Tag Free at 2 μg/mL (100 μL/well) can bind Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxinwith a linear range of 4-63 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Mouse VEGF164, Tag Free  at 2 μg/mL (100 μL/well) can bind Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxinwith a linear range of 2-31 ng/mL (Routinely tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Mouse VEGF164, His,Avitag  on SA Biosensor, can bind Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 42.4 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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