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Human GDF-15 / MIC-1 Protein, His Tag

Human GDF-15 / MIC-1 Protein, His Tag
别名: GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1
储存条件: -20℃ 
Human GDF-15 / MIC-1 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-GD5-H5149-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Human GDF-15 / MIC-1 Protein, His Tag

分子别名(Synonym)

GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1

表达区间及表达系统(Source)

Human GDF-15, His Tag  is expressed from E.coli cells. It contains AA Ala 197 - Ile 308 .

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 197 - Ile 308) Q99988-1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 14.2 kDa. The protein migrates as 15-16 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 30% ACN, 0.085% TFA. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human GDF-15, His Tag (Cat. No. GD5-H5149) SDS-PAGE gel

Human GDF-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human GDF-15, His Tag  at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag  with a linear range of 0.4-13 ng/mL (QC tested).

 

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