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Human IGFBP-7 Protein, His Tag

Human IGFBP-7 Protein, His Tag
别名: IBP7,IGFBP7
储存条件: -20℃ 
Human IGFBP-7 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IG7-H5240-50ug 50ug 现货 2900.00 2900
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熔点:
密度:
储存条件: -20℃

Human IGFBP-7 Protein, His Tag

分子别名(Synonym)

IBP7,IGFBP7

表达区间及表达系统(Source)

Human IGFBP-7, His Tagis expressed from human 293 cells . It contains AA Asp 30 - Leu 282 .

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 27.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IGFBP-7, His Tag (Cat. No. IG7-H5240) SDS-PAGE gel

Human IGFBP-7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IGFBP-7, His Tag  immobilized on CM5 Chip can bind Human C1q R1, His Tag with an affinity constant of 16.7 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

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