Biotinylated Human IL-6 Protein, epitope tag free, ultra sensitivity (primary amine labeling)

Biotinylated Human IL-6 Protein, epitope tag free, ultra sensitivity (primary amine labeling)

分子别名（Synonym）

IL6,Interleukin-6,BSF2,HSF,IFNB2

表达区间及表达系统（Source）

MABSol® Biotinylated Human IL-6, epitope tag free, primary amine labeling is expressed from human cells. It contains AA Val 30 - Met 212. It is the biotinylated form of ActiveMax® Human IL-6, Tag Free (IL6-H4218).

Predicted N-terminus: Val 30

Request for sequence

蛋白结构（Molecular Characterization）

The product does NOT contain any epitope tags. The protein has a calculated MW of 20.8 kDa. The protein migrates as 24-27 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.

标记（Biotinylation）

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Human IL-6, epitope tag free, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性（Bioactivity）-ELISA

Immobilized Human IL-6 R alpha, Fc Tagat 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6, epitope tag free, primary amine labelingwith a linear range of 1-20 ng/mL (QC tested).

Protocol

活性（Bioactivity）-BLI

Loaded Biotinylated Human IL-6, epitope tag free, primary amine labeling on SA Biosensor, can bind Human IL-6 R alpha, His Tag  with an affinity constant of 45.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性（Bioactivity）-Cell based assay

Biotinylated Human IL-6, epitope tag free, primary amine labeling stimulates proliferation of TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2532-0.4489 ng/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！