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| 别名: | KDR,CD309,FLK1,VEGFR,VEGFR2 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Mouse VEGF R2 / KDR Protein, Mouse IgG2a Fc Tag, low endotoxin
KDR,CD309,FLK1,VEGFR,VEGFR2
Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin is expressed from human 293 cells. It contains AA Ala 20 - Glu 762 .
Predicted N-terminus: Ala 20
Request for sequence
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 110.1 kDa. The protein migrates as 120-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 0.1 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
获取HPLC数据
Immobilized ActiveMax® Human VEGF165, Tag Free (HPLC-verified)at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 1-31 ng/mL (QC tested).
Protocol
Immobilized ActiveMax® Mouse VEGF120, Tag Freeat 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxinwith a linear range of 1-31 ng/mL (Routinely tested).
Protocol
Immobilized ActiveMax® Mouse VEGF164, Tag Free (HPLC-verified) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 2-31 ng/mL (Routinely tested).
Protocol
Loaded Biotinylated Mouse VEGF164, His,Avitag on SA Biosensor, can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxinwith an affinity constant of 1.33 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
