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Mouse Latent TGF-beta 1 / TGFB1 Protein, His Tag

Mouse Latent TGF-beta 1 / TGFB1 Protein, His Tag
别名: Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB
储存条件: -20℃ 
Mouse Latent TGF-beta 1 / TGFB1 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-TG1-M53245-50ug 50ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 Mouse Latent TGF-beta 1 / TGFB1 Protein, His Tag

分子别名(Synonym)

Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB

表达区间及表达系统(Source)

Mouse Latent TGFB1, His Tag s expressed from human 293 cells . It contains AA Leu 30 - Ser 390 ).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 43.2 kDa. The protein migrates as 12 kDa and 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally Trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

After reconstitution, this product is stable after storage at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse Latent TGFB1, His Tag (Cat. No. TG1-M5245) SDS-PAGE gel

Mouse Latent TGFB1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse Latent TGFB1, His Tagat 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free  with a linear range of 1-39 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Mouse Latent TGFB1, His Tag  at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free  with a linear range of 1-20 ng/mL (Routinely tested).

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