Human 4-1BB Ligand / TNFSF9 (71-254) Protein, His,Flag Tag (active trimer) (MALS verified)

Human 4-1BB Ligand / TNFSF9 (71-254) Protein, His,Flag Tag (active trimer) (MALS verified)

分子别名（Synonym）

4-1BB Ligand,TNFSF9,CD137L

表达区间及表达系统（Source）

Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified)  is expressed from human 293 cells . It contains AA Arg 71 - Glu 254 imer Design.

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 62.8 kDa. The protein migrates as 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) is more than 85% in HP-SEC, and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) at 2 μg/mL (100 μL/well) can bind Human 4-1BB, Fc Tag with a linear range of 0.2-3 ng/mL (QC tested).

Protocol

活性（Bioactivity）-BLI

Loaded Mouse 4-1BB, Fc Tag  on Protein A Biosensor, can bind Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) with an affinity constant of 18.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

活性（Bioactivity）-Cell based assay

Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.20-0.44 μg/mL (Routinely tested).

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