Human HVEM / TNFRSF14 Protein, Fc Tag

Human HVEM / TNFRSF14 Protein, Fc Tag

分子别名（Synonym）

TNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270

表达区间及表达系统（Source）

Human HVEM, Fc Tag  is expressed from human 293 cells. It contains AA Leu 39 - Val 202 ).

Predicted N-terminus: Leu 39

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 43.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human HVEM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性（Bioactivity）-ELISA

Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxinat 2 μg/mL (100 μL/well) can bind Human HVEM, Fc Tag with a linear range of 0.3-20 ng/mL (QC tested).

Protocol

Immobilized Human HVEM, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA (31-134), Fc,Avitag with a linear range of 0.156-5 μg/mL (Routinely tested).

Protocol

活性（Bioactivity）-BLI

Loaded Human HVEM, Fc Tag on Protein A Biosensor, can bind Human CD160, His Tag  with an affinity constant of 0.44 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely

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