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| 别名: | IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,Interleukin-17F,Cytokine ML-1 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human IL-17A&IL-17F Protein, Avitag™,His Tag
IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,Interleukin-17F,Cytokine ML-1
Biotinylated Human IL-17A&IL-17F, Avitag,His Tag s expressed from human 293 cells. It contains AA Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F).
Predicted N-terminus: Gly 24
Request for sequence
Biotinylated Human IL-17A&IL-17F, Avitag,His Tag, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 34.1 kDa. Subunit IL-17A&IL-17F is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The reducing (R) protein migrates as 40-43 kDa due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human IL-17A&IL-17F, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Anti-Human IL17A MAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17A&IL-17F, Avitag,His Tag with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Loaded Biotinylated Human IL-17A&IL-17F, Avitag,His Tag on SA Biosensor, can bind Human IL-17 RA, His Tag with an affinity constant of 352 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human IL-17A&IL-17F, Avitag,His Tag on SA Biosensor, can bind uman IL-17 RC, Fc Tag with an affinity constant of 217 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
