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Human HVEM / TNFRSF14 Protein, His Tag (MALS verified)

Human HVEM / TNFRSF14 Protein, His Tag (MALS verified)
别名: TNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270
储存条件: -20℃ 
Human HVEM / TNFRSF14 Protein, His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-HVM-H52E9-100ug 100ug 现货 2900.00 2900
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熔点:
密度:
储存条件: -20℃

Human HVEM / TNFRSF14 Protein, His Tag (MALS verified)

分子别名(Synonym)

TNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270

表达区间及表达系统(Source)

Human HVEM, His Tagressed from human 293 cells. It contains AA Leu 39 - Val 202 .

Predicted N-terminus: Leu 39

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 19.2 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human HVEM, His Tag (Cat. No. HVM-H52E9) SDS-PAGE gel

Human HVEM, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human HVEM, His Tag is more than 85% and the molecular weight of this protein is around 30-42 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin t 2 μg/mL (100 μL/well) can bind Human HVEM, His Tag  with a linear range of 0.02-0.3 μg/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human HVEM, His Tag at 5 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag  with a linear range of 0.2-2.5 μg/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Human HVEM, His Tag at 0.5 μg/mL (100 μL/well) can bind Human BTLA, Fc Tagwith a linear range of 0.078-2.5 μg/mL (Routinely tested).

 

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