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Human IGFBP-7 Protein, Fc Tag (MALS verified)

Human IGFBP-7 Protein, Fc Tag (MALS verified)
别名: IBP7,IGFBP7
储存条件: -20℃ 
Human IGFBP-7 Protein, Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IG7-H5259-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Human IGFBP-7 Protein, Fc Tag (MALS verified)

分子别名(Synonym)

IBP7,IGFBP7

表达区间及表达系统(Source)

Human IGFBP-7, Fc Tag  is expressed from human 293 cells . It contains AA Asp 30 - Leu 282.

Predicted N-terminus: Asp 30

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 52.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IGFBP-7, Fc Tag (Cat. No. IG7-H5259) SDS-PAGE gel

Human IGFBP-7, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

Human IGFBP-7, Fc Tag (Cat. No. IG7-H5259) MALS images

The purity of Human IGFBP-7, Fc Tagis more than 90% and the molecular weight of this protein is around 102-124 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human C1q R1, His Tag at 5 μg/mL (100 μL/well) can bind Human IGFBP-7, Fc Tag with a linear range of 0.6-20 ng/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IGFBP-7 Protein, Fc Tag immobilized on CM5 Chip can bind Human C1q R1 / CD93 Protein, His Tag (MALS verified) with an affinity constant of 7.29 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 

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