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| 别名: | PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human PDGF-BB Protein, Tag Free
PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis
Human PDGF-BB, Tag Free is expressed from E.coli cells. It contains AA Ser 82 - Thr 190
Predicted N-terminus: Met
Request for sequence
Human PDGF-BB, Tag Free is a disulfide-linked homodimeric polypeptide chain containing 2×109 amino acids, and has a calculated MW of 12.4 kDa. The reducing (R) protein migrates as a 14 kDa polypeptide and the non-reducing (NR) disulfide-linked homodimer migrates as a 27-32 kDa protein.
Less than 0.1 EU per μg by the LAL method.
>90% as determined by reducing (R) and non-reducing (NR) SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human PDGF-BB, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human PDGF-BB, Tag Freeat 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag with a linear range of 0.6-10 ng/mL (QC tested).
Protocol
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 0.5-20 ng/mL (Routinely tested).
