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Human IL-2 Protein, His Tag

Human IL-2 Protein, His Tag
别名: IL2,TCGF,lymphokine,Interleukin 2
储存条件: -20℃ 
Human IL-2 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IL2-H52H8-5 100ug 现货 0.00
ZY-IL2-H52H8-100ug 100ug 现货 3000.00 3000
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熔点:
密度:
储存条件: -20℃

 

Human IL-2 Protein, His Tag

分子别名(Synonym)

IL2,TCGF,lymphokine,Interleukin 2

表达区间及表达系统(Source)

Human IL-2, His Tag is expressed from human 293 cells . It contains AA Ala 21 - Thr 153.

Predicted N-terminus: Ala 21

Request for sequence

蛋白结构(Molecular Characterization)

20211231040108296292021123104011474316

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 17.3 kDa. The protein migrates as 18 kDa and 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-2, His Tag (Cat. No. IL2-H52H8) SDS-PAGE gel

Human IL-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-2 R alpha, Fc Tag  at 5 μg/mL (100 μL/well) can bind Human IL-2, His Tag  with a linear range of 0.3-2.5 ng/mL.

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag on SA Biosensor, can bind Human IL-2, His Tag  with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 

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