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| 别名: | TNFSF13B,BAFF,BLYS,CD257,DTL,TALL1,THANK,TNFSF20,ZTNF4,TALL-1 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 密度: | |
| 储存条件: | -20℃ |
Human BAFF / TNFSF13B / CD257 Protein, Fc Tag, active trimer (MALS verified)
TNFSF13B,BAFF,BLYS,CD257,DTL,TALL1,THANK,TNFSF20,ZTNF4,TALL-1
Human BAFF, Fc Tag, active trimer is expressed from human 293 cells. It contains AA Glu 140 - Leu 285 trimer Design.
Predicted N-terminus: Pro
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 76.7 kDa. The protein migrates as 120-140 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human BAFF, Fc Tag, active trimer on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human BAFF, Fc Tag, active trimer is more than 90% and the molecular weight of this protein is around 150-170 kDa verified by SEC-MALS.
Report
Immobilized Human BCMA, Mouse IgG2a Fc Tag, low endotoxin at 1 μg/mL (100 μL/well) can bind Human BAFF, Fc Tag, active trimer with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Human BAFF, Fc Tag, active trimerat 1 μg/mL (100 μL/well) can bind Biotinylated Human TACI, Fc,Avitag with a linear range of 0.002-0.05 μg/mL (Routinely tested).
Protocol
Immobilized Human BAFF, Fc Tag, active trimer at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse BAFFR, Fc,Avitag with a linear range of 0.4-25 ng/mL (Routinely tested).
Protocol
Immobilized Human BAFF, Fc Tag, active trimer n CM5 Chip can bind Human TACI/TNFRSF13B, Fc Tag with an affinity constant of 0.658 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Immobilized Human BAFF, Fc Tag, active trime captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human BAFFR, Llama IgG2b Fc Tag, low endotoxin with an affinity constant of 44.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Human BAFF, Fc Tag, active trimer on Protein A Biosensor, can bind Biotinylated Human BCMA, His,Avitag with an affinity constant of 0.69 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Human BAFF, Fc Tag, active trimer stimulates proliferation of mouse spleen cells. The EC50 for this effect is 4.91-9.81 ng/mL (Routinely tested)
