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| 别名: | IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IGF-I R / CD221 Protein, His Tag (MALS verified)
IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216
Human IGF-I R, His Tag is expressed from human 293 cells . It contains AA Glu 31 - Asn 932
Predicted N-terminus: Glu 31
Request for sequence
This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.0 kDa and 23.8 kDa respectively. The protein migrates as 35-45 kDa (partial β chain), 110-120 kDa (α chain) and 130 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IGF-I R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human IGF-I R, His Tag is more than 90% and the molecular weight of this protein is around 252-308 kDa verified by SEC-MALS.
Report
Immobilized Human IGF-I R, His Tag at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 0.01-0.625 μg/mL (QC tested).
Protocol
Human IGF-I R, His Tagimmobilized on CM5 Chip can bind Biotinylated Human IGF-I, His,Avitagwith an affinity constant of 68 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human IGF-I R, His Tag captured on CM5 chip via anti-His antibody can bind Human IGF-I, Fc Tag with an affinity constant of 0.189 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human IGF-I R, His Tag immobilized on CM5 Chip can bind Biotinylated Human IGF-II, His,Avitag with an affinity constant of 51.2 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
