Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)

Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)

分子别名（Synonym）

IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG

表达区间及表达系统（Source）

Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag  is expressed from human 293 cells. It contains AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG).

Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)

Request for sequence

蛋白结构（Molecular Characterization）

Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 29.9 kDa (IL-2RB) and 34.1 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 40-44 kDa and 60-65 kDa respectively due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70^°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is more than 85% and the molecular weight of this protein is around 90-115 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Human IL-2, Tag Freeat 5 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag with a linear range of 20-78 ng/mL (QC tested).

Protocol

活性（Bioactivity）-BLI

Loaded Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag on NTA Biosensor, can bind Human IL-2, Tag Free  with an affinity constant of 1.42 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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