Biotinylated Human IL-13 Protein, His,Avitag™

Biotinylated Human IL-13 Protein, His,Avitag™

分子别名（Synonym）

IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600,Interleukin-13

表达区间及表达系统（Source）

Biotinylated Human IL-13, His,Avitag  is expressed from human 293 cells. It contains AA Gly 21 - Asn 132 .

Predicted N-terminus: Gly 21

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 15.9 kDa. The protein migrates as 17 kDa and 28-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Human IL-13, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性（Bioactivity）-ELISA

Immobilized Human IL-13 R alpha 2, Fc Tagat 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-13, His,Avitag with a linear range of 0.2-5 ng/mL (QC tested).

Protocol

Immobilized Biotinylated Human IL-13, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin  precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human IL-13 Antibody, Human IgG4 with a linear range of 0.3-20 ng/mL (Routinely tested).

Protocol

活性（Bioactivity）-BLI

Loaded Biotinylated Human IL-13, His,Avitag  on SA Biosensor, can bind Human IL-13 R alpha 2, His Tag with an affinity constant of 6.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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