Rat OX40 Ligand / TNFSF4 Protein, His Tag (active trimer) (MALS verified)

Rat OX40 Ligand / TNFSF4 Protein, His Tag (active trimer) (MALS verified)

分子别名（Synonym）

OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L

表达区间及表达系统（Source）

Rat OX40 Ligand, His Tag is expressed from human 293 cells  It contains AA Gln 49 - Leu 199.

Predicted N-terminus: His

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 53.8 kDa. The protein migrates as 58-68 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70^°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Rat OX40 Ligand, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%

The purity of Rat OX40 Ligand, His Tagis more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Rat OX40, Fc Tag at 2 μg/mL (100 μL/well) can bind Rat OX40 Ligand, His Tag with a linear range of 20-78 ng/mL (QC tested).

Protocol

Immobilized Rat OX40 Ligand, His Tag at 2 μg/mL (100 μL/well) can bind Rat OX40, Fc Tag with a linear range of 0.2-5 ng/mL (Routinely tested).

 

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