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Biotinylated Monoclonal Anti-IFNγ antibody, Mouse IgG1 (13E6H6) (MALS verified)
Biotinylated Monoclonal Anti-IFNγ antibody, Mouse IgG1 is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with IFNγ. It is the chemically biotin labeled form of Monoclonal Anti-IFNγ antibody, Mouse IgG1 .
Mouse IgG1/kappa
This product is a specific antibody specifically reacts with IFN-γ, Human. No cross-reactivity is detected with other human cytokines, including IL-2, IL-4, IL-6, IL-10, GM-CSF and TNF-alpha.
ELISA, LFA
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Less than 1.0 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Monoclonal Anti-IFNγ antibody, Mouse IgG1 (13E6H6) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Monoclonal Anti-IFNγ antibody, Mouse IgG1 (13E6H6) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Report
Immobilized ActiveMax® Human IFN-gamma, Tag Free can bind Biotinylated Monoclonal Anti-IFNγ antibody, Mouse IgG1 (13E6H6) with a linear range of 0.05-3.125 ng/mL (QC tested).
Protocol
Biotinylated Monoclonal Anti-IFNγ antibody, Mouse IgG1 (13E6H6) captured on CM5 chip via anti-mouse antibodies surface can bind ActiveMax® Human IFN-gamma, Tag Free with an affinity constant of 1.28 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Loaded Biotinylated Monoclonal Anti-IFNγ antibody, Mouse IgG1 (13E6H6) on AMC Biosensor, can bind ActiveMax® Human IFN-gamma, Tag Free ith an affinity constant of 0.268 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
