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| 别名: | IL4,BCGF1,BSF1 | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
ActiveMax® Human IL-4 Protein, Tag Free
IL4,BCGF1,BSF1
ActiveMax® Human IL-4, Tag Free is expressed from human 293 cells . It contains AA His 25 - Ser 153.
Predicted N-terminus: His 25
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 15.0 kDa. The protein migrates as 15 kDa and 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 0.1 EU per μg by the LAL method.
The sterility testing was performed by membrane filtration method described in USP <71> and Eur. Ph. 2.6.1.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human IL-4, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized ActiveMax® Human IL-4, Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4 R alpha, Avitag,His Tagwith a linear range of 2-78 ng/mL (QC tested).
Protocol
Immobilized ActiveMax® Human IL-4, Tag Freeat 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
ActiveMax® Human IL-4, Tag Free stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.27-0.49 ng/mL (Routinely tested).
