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Biotinylated Human 4-1BB Ligand / TNFSF9 Protein, Fc,Avitag™

Biotinylated Human 4-1BB Ligand / TNFSF9 Protein, Fc,Avitag™
别名: 4-1BB Ligand,TNFSF9,CD137L
储存条件: -20℃ 
Biotinylated Human 4-1BB Ligand / TNFSF9 Protein, Fc,Avitag™
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-41L-H82F9-25ug 25ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Biotinylated Human 4-1BB Ligand / TNFSF9 Protein, Fc,Avitag™

分子别名(Synonym)

4-1BB Ligand,TNFSF9,CD137L

表达区间及表达系统(Source)

Biotinylated Human 4-1BB Ligand, Fc,Avitag is expressed from human 293 cells. It contains AA Ala 50 - Glu 254 .

Predicted N-terminus: Ala 50

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 50.2 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Biotinylated Human 4-1BB Ligand, Fc,Avitag (Cat. No. 41L-H82F9) SDS-PAGE gel

Biotinylated Human 4-1BB Ligand, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human 4-1BB, Fc Tag at 0.1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB Ligand, Fc,Avitag ith a linear range of 0.4-13 ng/mL (QC tested).

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