Human GFR alpha-like Protein, Fc Tag

Human GFR alpha-like Protein, Fc Tag

分子别名（Synonym）

GFR alpha like,GFR alpha-like,GFRAL,GRAL

表达区间及表达系统（Source）

Human GFR alpha-like, Fc Tag is expressed from human 293 cells . It contains AA Ser 19 - Glu 351 .

Predicted N-terminus: Ser 19

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 64.2 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human GFR alpha-like, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性（Bioactivity）-ELISA

Immobilized Human GDF-15, His Tagat 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag  with a linear range of 0.4-25 ng/mL (QC tested).

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