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Mouse LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified)

Mouse LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified)
别名: LRRC32 & TGF-beta 1,LRRC32&TGFB1
储存条件: -20℃ 
Mouse LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-GA1-M52W2-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Mouse LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified)

 

分子别名(Synonym)

LRRC32 & TGF-beta 1,LRRC32&TGFB1

表达区间及表达系统(Source)

Mouse LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Freeis expressed from human 293 cells. It contains AA Ile 18 - Asn 628 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1).

 

Request for sequence

蛋白结构(Molecular Characterization)

Mouse LRRC32&TGFB1 Heterotrimer Protein, His Tag&Tag Free (the molar ratio of LRRC32 & TGF-beta 1 equals 1:2) is produced by co-expression of LRRC32 and TGF-beta 1, which has a calculated MW of 68.7 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature TGF-beta 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and TGF-beta 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 80 kDa (LRRC32), 40 kDa (LAP) and 14 kDa (mature TGF-beta 1) due to glycosylation respectively.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (Cat. No. GA1-M52W2) SDS-PAGE gel

Mouse LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Mouse LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free is more than 90% and the molecular weight of this protein is around 155-170 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free at 1 μg/mL (100 μL/well) can bind Purified anti-mouse LAP (TGF-β1) Antibody with a linear range of 0.2-1 ng/mL (QC tested).

 

 

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