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Mouse CD40 Ligand / TNFRSF5 Protein, His,Flag Tag (active trimer) (MALS verified)

Mouse CD40 Ligand / TNFRSF5 Protein, His,Flag Tag (active trimer) (MALS verified)
别名: CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
储存条件: -20℃ 
Mouse CD40 Ligand / TNFRSF5 Protein, His,Flag Tag (active trimer) (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-CDL-M52D5-100ug 100ug 现货 2900.00 2900
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熔点:
密度:
储存条件: -20℃

Mouse CD40 Ligand / TNFRSF5 Protein, His,Flag Tag (active trimer) (MALS verified)

分子别名(Synonym)

CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39

表达区间及表达系统(Source)

Mouse CD40 Ligand, His,Flag Tag is expressed from human 293 cells. It contains AA Gly 115 - Leu 260.

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 50.5 kDa. The protein migrates as 45-55 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse CD40 Ligand, His,Flag Tag (Cat. No. CDL-M52D5) SDS-PAGE gel

Mouse CD40 Ligand, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Mouse CD40 Ligand, His,Flag Tag is more than 95% and the molecular weight of this protein is around50-60kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse CD40 Ligand, His,Flag Tag t 2 μg/mL (100 μL/well) can bind Mouse CD40, Fc Tag  with a linear range of 1-32 ng/mL (QC tested).

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