Biotinylated Human CD40 Ligand / TNFSF5 Protein, Avitag™,Fc Tag

Biotinylated Human CD40 Ligand / TNFSF5 Protein, Avitag™,Fc Tag

分子别名（Synonym）

CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39

表达区间及表达系统（Source）

Biotinylated Human CD40 Ligand, Avitag,Fc Tag is expressed from human 293 cells. It contains AA Met 113 - Leu 261 .

Predicted N-terminus: Gly

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.

The protein has a calculated MW of 45.0 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Human CD40 Ligand, Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性（Bioactivity）-ELISA

Immobilized Human CD40, His Tag  at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD40 Ligand, Avitag,Fc Tagwith a linear range of 1-31 ng/mL (QC tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！