Human NRG1 Beta 1 Protein, Fc Tag

Human NRG1 Beta 1 Protein, Fc Tag

分子别名（Synonym）

NRG1 Beta 1,HRG1 beta 1,Neuregulin-1 beta 1,Neuregulin1 beta 1,NRG1,ARIA,GGF,HGL,HRGA,NDF,SMDF

表达区间及表达系统（Source）

Human NRG1 Beta 1, Fc Tagis expressed from human 293 cells . It contains AA Ser 2 - Lys 246

Predicted N-terminus: Pro

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 53.2 kDa. The protein migrates as 65-80 kDa and 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human NRG1 Beta 1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性（Bioactivity）-ELISA

Immobilized Human ErbB3, His Tag at 2 μg/mL (100 μL/well) can bind Human NRG1 Beta 1, Fc Tag with a linear range of 0.5-8 ng/mL (QC tested).

Protocol

Immobilized Human ErbB4, His Tag at 2 μg/mL (100 μL/well) can bind Human NRG1 Beta 1, Fc Tag with a linear range of 0.5-8 ng/mL (Routinely tested).

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