Human CCR8 Full Length Protein-VLP (HEK293)

Human CCR8 Full Length Protein-VLP (HEK293)

分子别名（Synonym）

CC chemokine receptor 8,CD198,ChemR1,CKRL1,CKR-L1,CKRL1,CY6,GPRCL1,GPRCY6,GPR-CY6,TER-1

表达区间及表达系统（Source）

Human CCR8 Full Length Protein-VLP  is expressed from human 293 cells. It contains AA Asp 2 - Leu 355

Predicted N-terminus: Asp

蛋白结构（Molecular Characterization）

Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrance Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in PBS, pH 7.4 with trehalose as protectant.

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

存储（Storage）

 

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;

2. -70^°C for 12 months under sterile conditions.

*The isotype control of empty/mock VLP is sold separately and not included in protein, you can follow this link for product information.

 

活性（Bioactivity）-ELISA

Immobilized Human CCR8 Full Length Protein-VLP  at 10 μg/mL (100 μL/well) can bind Anti-CCR8 Antibody, Human IgG1 with a linear range of 0.078-2.5 μg/mL (QC tested).

Protocol

 

均一性（Identity）-DLS

The mean peak Radius of VLP is 55-75 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

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