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| 别名: | IL-2 R beta & IL-2 R alpha & IL-2 R gamma | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, His Tag&Twin Strep Tag (MALS verified)
IL-2 R beta & IL-2 R alpha & IL-2 R gamma
Human IL-2RB&IL-2RA&IL-2RG, His Tag&Twin Strep Tagis expressed from human 293 cells . It contains AA Ala 27 - Asp 239 (IL-2RB) & Glu 22 - Ser 212 (IL-2RA) & Leu 23 - Asn 254 (IL-2RG) .
Predicted N-terminus: Ala 27
Human IL-2RB&IL-2RA&IL-2RG, His Tag&Twin Strep Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 29.9 kDa (IL-2RB) & 55.8 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a his tag at the C-terminus and subunit IL-2RA&IL-2RG is fused with a Twin-Strep Tag at the C-terminus. The reducing (R) protein migrates as 37 kDa, 42 kDa and 90 kDa respectively due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-2RB&IL-2RA&IL-2RG, His Tag&Twin Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human IL-2RB&IL-2RA&IL-2RG, His Tag&Twin Strep Tagis more than 90% and the molecular weight of this protein is around 117-137 kDa verified by SEC-MALS.
Report
Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RA&IL-2RG, His Tag&Twin Strep Tag with a linear range of 0.01-0.313 μg/mL (QC tested).
Protocol
Human IL-2RB&IL-2RA&IL-2RG, His Tag&Twin Strep Tag captured on CM5 chip via anti-His antibody can bind Human IL-2, Tag Free with an affinity constant of 56.9 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
