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| 别名: | TNFSF13B,BAFF,BLYS,CD257,DTL,TALL1,THANK,TNFSF20,ZTNF4,TALL-1 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human BAFF / TNFSF13B / CD257 Protein, Avitag™,Fc Tag (active trimer) (MALS verified)
TNFSF13B,BAFF,BLYS,CD257,DTL,TALL1,THANK,TNFSF20,ZTNF4,TALL-1
Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified) is expressed from human 293 cells. It contains AA Ala 134 - Leu 285.
Predicted N-terminus: Gly
Request for sequence
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 44.9 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified)is more than 85% in HP-SEC, and the molecular weight of this protein is around 280-320 kDa verified by SEC-MALS.
Report
Immobilized Human BCMA, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified)with a linear range of 1.95-15.6 ng/mL (QC tested).
Protocol
Immobilized Human TACI/TNFRSF13B, Fc Tagat 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified)
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Llama IgG2b Fc Tag, low endotoxin Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8129 μg/mL (Routinely tested).
Protocol
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Fc Tag : Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified)binding reactions. The half maximal inhibitory concentration (IC50) is 0.4692 μg/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified)on SA Chip can bind Human TACI/TNFRSF13B, Fc Tag with an affinity constant of 0.988 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Immobilized Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verifiedon SA Chip can bind Human BAFFR, Fc Tag with an affinity constant of 26.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified) on SA Biosensor, can bind Human BCMA, His Tagwith an affinity constant of 1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
