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Cynomolgus APRIL / TNFSF13 Protein, His,Flag Tag, active trimer (MALS verified)

Cynomolgus APRIL / TNFSF13 Protein, His,Flag Tag, active trimer (MALS verified)
别名: APRIL,TNFSF13,TALL-2,TRDL-1,CD256,TALL2,ZTNF2
储存条件: -20℃ 
Cynomolgus APRIL / TNFSF13 Protein, His,Flag Tag, active trimer (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-APL-C52D2-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Cynomolgus APRIL / TNFSF13 Protein, His,Flag Tag, active trimer (MALS verified)

分子别名(Synonym)

APRIL,TNFSF13,TALL-2,TRDL-1,CD256,TALL2,ZTNF2

表达区间及表达系统(Source)

Cynomolgus APRIL, His,Flag Tag  is expressed from human 293 cells . It contains AA Gln 111 - Leu 250 trimer Design.

Predicted N-terminus: His

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 50.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Cynomolgus APRIL, His,Flag Tag (Cat. No. APL-C52D2) SDS-PAGE gel

Cynomolgus APRIL, His,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Cynomolgus APRIL, His,Flag Tag is more than 90% and the molecular weight of this protein is around 57-69 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Cynomolgus APRIL, His,Flag Tag at 1 μg/mL (100 μL/well) can bind Human TACI (R119A, R122A), Fc Tagwith a linear range of 2-13 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Cynomolgus APRIL, His,Flag Tag at 1 μg/mL (100 μL/well) can bind Human BCMA, Fc Tag (HPLC verified) with a linear range of 0.1-1 ng/mL (Routinely tested).

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