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| 别名: | TNFRSF10A,TRAIL-R1,CD261,APO2,DR4 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human TRAIL R1 / DR4 / TNFRSF10A Protein, Fc Tag
TNFRSF10A,TRAIL-R1,CD261,APO2,DR4
Human TRAIL R1, Fc Tag is expressed from human 293 cells. It contains AA Ala 24 - Asn 239
Predicted N-terminus: Ala 24
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human TRAIL R1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human TRAIL R1, Fc Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag with a linear range of 4-16 ng/mL (QC tested).
Protocol
Loaded Human TRAIL R1, Fc Tag on AHC Biosensor, can bind Human TRAIL, His Tag with an affinity constant of 0.891 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human TRAIL, His Tag on HIS1K Biosensor, can bind Human TRAIL R1, Fc Tag with an affinity constant of 2.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
