-
专业包装 正品保证
-
快乐服务 售后无忧
-
会员特权 优惠不断
-
个人信息 严格保护
| 别名: | CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
|
| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
|---|
| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Human LIGHT / TNFSF14 Protein, Fc Tag (active trimer) (MALS verified)
CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML
Human LIGHT, Fc Tag (active trimer) (MALS verified)is expressed from human 293 cells t contains AA Asp 74 - Val 240 trimer Design.
Predicted N-terminus: Pro
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 82.6 kDa. The protein migrates as 116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human LIGHT, Fc Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human LIGHT, Fc Tag (active trimer) (MALS verified)is more than 90% in HP-SEC, and the molecular weight of this protein is around 180-200 kDa verified by SEC-MALS.
Report
Immobilized Human HVEM, His Tag at 5 μg/mL (100 μL/well) can bind Human LIGHT, Fc Tag (active trimer) (MALS verified) with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Human LIGHT, Fc Tag (active trimer) (MALS verified) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.29-2.38 ng/mL (Routinely tested).
