Biotinylated Mouse VEGF164 Protein, His,Avitag™

Biotinylated Mouse VEGF164 Protein, His,Avitag™

分子别名（Synonym）

RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

表达区间及表达系统（Source）

Biotinylated Mouse VEGF164, His,Avitag  is expressed from human 293 cells. It contains AA Ala 27 - Arg 190 

Predicted N-terminus: His

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 22.5 kDa. As a result of different glycosylation, the protein migrates as 28-33 kDa under reducing (R) condition, and 46-60 kDa under non-reducing (NR) condition (SDS-PAGE).

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Mouse VEGF164, His,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性（Bioactivity）-ELISA

Immobilized VEGFR1/R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF164, His,Avitag with a linear range of 0.02-0.5 ng/mL (QC tested).

Protocol

Immobilized Human VEGF R1, His Tag  at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse VEGF164, His,Avitag with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

活性（Bioactivity）-BLI

Loaded Biotinylated Mouse VEGF164, His,Avitag on SA Biosensor, can bind Mouse VEGF R1, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 42.4 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Loaded Biotinylated Mouse VEGF164, His,Avitag  on SA Biosensor, can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin  with an affinity constant of 1.33 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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