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| 别名: | RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 密度: | |
| 储存条件: | -20℃ |
ActiveMax® Human VEGF110 Protein, Tag Free (MALS verified)
RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
ActiveMax® Human VEGF110, Tag Free is expressed from human 293 cells . It contains AA Ala 27 - Arg 136.
Predicted N-terminus: Ala 27
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 12.7 kDa. The protein migrates as 16-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
This product does not contain a heparin binding domain, and therefore doesn't bind with heparin.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
ActiveMax® Human VEGF110, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of ActiveMax® Human VEGF110, Tag Free is more than 85% and the molecular weight of this protein is around 28-38 kDa verified by SEC-MALS.
Report
Immobilized ActiveMax® Human VEGF110, Tag Free (HPLC-verified) at 0.005 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag with a linear range of 2-16ng/mL (QC tested).
Protocol
ActiveMax® Human VEGF110, Tag Free (HPLC-verified)stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The EC50 for this effect is 5.06-11.59 ng/mL (Routinely tested).
