Human HGF R / c-MET Protein, Fc Tag (MALS verified)

Human HGF R / c-MET Protein, Fc Tag (MALS verified)

分子别名（Synonym）

MET,AUTS9,HGFR,RCCP2,c-Met

表达区间及表达系统（Source）

Human HGF R, Fc Tag is expressed from human 293 cells. It contains AA Glu 25 - Thr 932 

Predicted N-terminus: Glu 25 (α chain) & Ser 308 (β chain Fc chimera)

Request for sequence

蛋白结构（Molecular Characterization）

Human HGF R, Fc Tag is fused with Fc fragment of human IgG1 at the C-terminus. The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.5 kDa (α chain) and 95.3 kDa (β chain Fc chimera). The protein migrates as 40-45 kDa (α chain) and 106-116 kDa (β chain Fc chimera) under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human HGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human HGF R, Fc Tag is more than 90% and the molecular weight of this protein is around 280-340 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Human HGF, His Tag at 2 μg/mL (100 μL/well) can bind Human HGF R, Fc Tag with a linear range of 0.6-20 ng/mL (QC tested).

Protocol

Immobilized Human HGF R, Fc Tag at 5 μg/mL (100 μL/well) can bind Human HGF, His Tag  with a linear range of 5-78 ng/mL (Routinely tested).

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