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| 别名: | IL4R,CD124,IL4RA | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-4 R alpha / CD124 Protein, His Tag (MALS verified)
IL4R,CD124,IL4RA
Human IL-4 R alpha, His Tag is expressed from human 293 cells . It contains AA Met 26 - His 232.
Predicted N-terminus: Met 26
Request for sequence
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 24.6 kDa. The protein migrates as 36-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-4 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-4 R alpha, His Tagis more than 85% and the molecular weight of this protein is around 32-48 kDa verified by SEC-MALS.
Report
Immobilized Human IL-4 R alpha, His Tag at 5μg/mL (100 μL/well) can bind Biotinylated Human IL-4, Avitag,His Tagwith a linear range of 2-16 ng/mL (QC tested).
Protocol
Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, His Tag: Biotinylated Human IL-4, Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.505 μg/mL (Routinely tested).
