Human IL-10 Protein, His Tag_细胞生物学_生命科学

Human IL-10 Protein, His Tag

别名:	CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10
		储存条件:	-20℃

货号	规格	可用库存	销售价(RMB)	您的折扣价(RMB)	购买数量
ZY-IL0-H4248-20ug	20ug	现货	~~1980.00~~	1980

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熔点:
密度:
储存条件:	-20℃

Human IL-10 Protein, His Tag

分子别名（Synonym）

CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10

表达区间及表达系统（Source）

Human IL-10, His Tag is expressed from human 293 cells . It contains AA Ser 19 - Asn 178.

Predicted N-terminus: His

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IL-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性（Bioactivity）-ELISA

Immobilized Human IL-10, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc,Avitag with a linear range of 0.2-10 ng/mL (QC tested).

Protocol

活性（Bioactivity）-Cell based assay

Human IL-10, His Tag inhibits LPS-induced secretion of IL-6 by RAW264.7 cells. The EC50 for this effect is 0.11-0.24 ng/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

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精细化学

分析色谱

材料科学

生命科学

标准品

Human IL-10 Protein, His Tag

分子别名（Synonym）

CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10

表达区间及表达系统（Source）

Human IL-10, His Tag is expressed from human 293 cells . It contains AA Ser 19 - Asn 178.

Predicted N-terminus: His

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IL-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性（Bioactivity）-ELISA

Immobilized Human IL-10, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc,Avitag with a linear range of 0.2-10 ng/mL (QC tested).

Protocol

活性（Bioactivity）-Cell based assay

Human IL-10, His Tag inhibits LPS-induced secretion of IL-6 by RAW264.7 cells. The EC50 for this effect is 0.11-0.24 ng/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

精细化学

分析色谱

材料科学

生命科学

标准品

Human IL-10 Protein, His Tag

分子别名（Synonym）

CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10

表达区间及表达系统（Source）

Human IL-10, His Tag is expressed from human 293 cells . It contains AA Ser 19 - Asn 178. Predicted N-terminus: His Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IL-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性（Bioactivity）-ELISA

Immobilized Human IL-10, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc,Avitag with a linear range of 0.2-10 ng/mL (QC tested). Protocol

活性（Bioactivity）-Cell based assay

Human IL-10, His Tag inhibits LPS-induced secretion of IL-6 by RAW264.7 cells. The EC50 for this effect is 0.11-0.24 ng/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

Human IL-10, His Tag is expressed from human 293 cells . It contains AA Ser 19 - Asn 178.

Predicted N-terminus: His

Request for sequence

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

Immobilized Human IL-10, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc,Avitag with a linear range of 0.2-10 ng/mL (QC tested).

Protocol