Human IL-1RL1 / ST2 Protein, His Tag

Human IL-1RL1 / ST2 Protein, His Tag

分子别名（Synonym）

IL1RL1,DER4,FIT-1,IL33R,ST2,ST2L,ST2V,T1

表达区间及表达系统（Source）

Human IL-1RL1, His Tag  is expressed from human 293 cells. It contains AA Lys 19 - Phe 328 .

Predicted N-terminus: Lys 19

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.9 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human IL-1RL1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

 

活性（Bioactivity）-ELISA

Immobilized Human IL-1RL1, His Tagat 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-33, His,Avitag  with a linear range of 0.02-0.313 μg/mL (QC tested).

Protocol

Immobilized human IL33 at 5 μg/mL (100 μL/well) can bind Human IL-1RL1, His Tag with a linear range of 0.125-0.5 μg/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！