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| 别名: | CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human IL-17 RA / CD217 Protein, Fc,Avitag™
CD217,CDw217,IL-17RA,IL17R,CANDF5,hIL-17R
Biotinylated Human IL-17 RA, Fc,Avitag is expressed from human 293 cells . It contains AA Leu 33 - Trp 320.
Predicted N-terminus: Leu 33
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 61.9 kDa. As a result of glycosylation, the protein migrates as 80-115 kDa under reducing (R) condition, and 150-200 kDa under non-reducing (NR) condition (SDS-PAGE).
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human IL-17 RA, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
获取HPLC数据
Immobilized Human IL17A, Tag Freeat 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag with a linear range of 2-125 ng/mL (QC tested).
Protocol
Immobilized Human IL-17F , His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
Loaded Biotinylated Human IL-17 RA, Fc,Avitag on SA Biosensor, can bind Human IL17A, Tag Free with an affinity constant of 1.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
