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Human FGF R2 (IIIb) protein, Fc Tag (MALS verified)

Human FGF R2 (IIIb) protein, Fc Tag (MALS verified)
别名: FGF R2 (IIIb),FGFR2B,FGFR2
储存条件: -20℃ 
Human FGF R2 (IIIb) protein, Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-FGB-H5256-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 Human FGF R2 (IIIb) protein, Fc Tag (MALS verified)

分子别名(Synonym)

FGF R2 (IIIb),FGFR2B,FGFR2

表达区间及表达系统(Source)

Human FGF R2 (IIIb), Fc Tag  is expressed from human 293 cells. It contains AA Pro 154 - Leu 358 

Predicted N-terminus: Pro 154

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 49.4 kDa. The protein migrates as 65-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) SDS-PAGE gel

Human FGF R2 (IIIb), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human FGF R2 (IIIb), Fc Tagis more than 90% and the molecular weight of this protein is around 119-145 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human FGF basic, Tag Free at 5 μg/mL (100 μL/well) can bind Human FGF R2 (IIIb), Fc Tag  with a linear range of 1-16 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Biotinylated Human FGF basic, Avitag,His Tag t 1 μg/mL (100 μL/well) on streptavidin  precoated (0.5 μg/well) plate can bind Human FGF R2 (IIIb), Fc Tag with a linear range of 2-31 ng/mL (Routinely tested).

Protocol

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