Human VEGF R2 / KDR Protein, Fc Tag (MALS verified)

Human VEGF R2 / KDR Protein, Fc Tag (MALS verified)

分子别名（Synonym）

KDR,CD309,FLK1,VEGFR,VEGFR2

表达区间及表达系统（Source）

Human VEGF R2, Fc Tag  is expressed from human 293 cells. It contains AA Ala 20 - Glu 764 .

Predicted N-terminus: Ala 20

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 109.7 kDa. The protein migrates as 140-170 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70^°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human VEGF R2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human VEGF R2, Fc Tag  is more than 90% and the molecular weight of this protein is around 256-313 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Human VEGF R2, Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-VEGFR2 Antibody, Mouse IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

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