Human EGFR-coupled Magnetic Beads

Human EGFR-coupled Magnetic Beads

Background/背景

The biotinylated EGFR protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-EGFR antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This EGFR coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.

Application/应用

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

Reconstitution/重构方法

See Certificate of Analysis (CoA) for detailed instruction.

Storage/存储

Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

 

Assay Principles/原理

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

Antibody Purification:

1. Resuspend the lyophilized beads by adding the buffer of choice.

2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

 

 

Typical Data/典型数据

Immobilized 35 μg EGFR protein to 1 mg Beads, can bind the Anti-EGFR Antibody (Cetuximab) with an EC50 of 0.737 μg/mL (QC tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！