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Biotinylated Human CX3CL1 Protein, His Tag, ultra sensitivity (primary amine labeling)

Biotinylated Human CX3CL1 Protein, His Tag, ultra sensitivity (primary amine labeling)
别名: CX3CL1,Fractalkine
储存条件: -20℃ 
Biotinylated Human CX3CL1 Protein, His Tag, ultra sensitivity (primary amine labeling)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-CX1-H8221-25ug 25ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Biotinylated Human CX3CL1 Protein, His Tag, ultra sensitivity (primary amine labeling)

CX3CL1,Fractalkine

表达区间及表达系统(Source)

Biotinylated Human CX3CL1, His Tag, primary amine labeling  is expressed from human 293 cells . It contains AA Gln 25 - Gln 341  is the biotinylated form of Human CX3CL1, His Tag .

Predicted N-terminus: Gln 25

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.5 kDa. The protein migrates as 50-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Biotinylated Human CX3CL1, His Tag, primary amine labeling (Cat. No. CX1-H8221) SDS-PAGE gel

Biotinylated Human CX3CL1, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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