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Canine IL-17A / CTLA-8 Protein, His Tag

Canine IL-17A / CTLA-8 Protein, His Tag
别名: IL-17A,Interleukin-17A,CTLA-8,IL-17
储存条件: -20℃ 
Canine IL-17A / CTLA-8 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILA-C52H7-100ug 100ug 现货 3800.00 3800
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熔点:
密度:
储存条件: -20℃

 

Canine IL-17A / CTLA-8 Protein, His Tag

分子别名(Synonym)

IL-17A,Interleukin-17A,CTLA-8,IL-17

表达区间及表达系统(Source)

Canine IL-17A, His Tag  is expressed from human 293 cells . It contains AA Gly 26 - Ala 155 .

Predicted N-terminus: Gly 26

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 17.0 kDa. The protein migrates as 18 kDa and 22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by reduced SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Canine IL-17A, His Tag (Cat. No. ILA-C52H7) SDS-PAGE gel

Canine IL-17A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Monoclonal Anti-Human IL17A Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Canine IL-17A, His Tag with a linear range of 5-39 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Canine IL-17A, His Tagat 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag  with a linear range of 4-63 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Canine IL-17A, His Tag  at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (MALS verified)with a linear range of 0.1-4 ng/mL (Routinely tested).

 

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