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| 别名: | IL12,p70,Interleukin-12 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag
IL12,p70,Interleukin-12
Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tagis expressed from human 293 cells . It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A) .
Predicted N-terminus: Ile 23 (IL12B) & Arg 23 (IL12A)
Request for sequence
Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.6 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with polyhistidine tag at the C-terminus and subunit IL-12A is fused with flag tag at the C-terminus. The reducing (R) heterodimer protein migrates as 45 kDa (IL12B) and 38-43 kDa (IL12A) respectively due to glyosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag at 0.5 μg/mL (100 μL/well) can bind Anti-IL23A & IL12B MAb (P40 domain) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-12 R beta 1, Fc,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag with a linear range of 0.039-0.625 μg/mL (Routinely tested).
Protocol
Human IL-12 R beta 1, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tagwith an affinity constant of 2.06 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Anti-IL23A & IL12B MAb (P40 domain) on Protein A Biosensor, can bind Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag with an affinity constant of 1.07 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag on HIS1K Biosensor, can bind Human IL-12 R beta 1, Fc Tag with an affinity constant of 92.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tagstimulates the proliferation of PHA-P activated PBMC. The ED50 for this effect is 0.05 ng/mL (Routinely tested).
