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| 别名: | IL-17F,Interleukin-17F,Cytokine ML-1 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-17F Protein, His Tag
IL-17F,Interleukin-17F,Cytokine ML-1
Human IL-17F, His Tag is expressed from human 293 cells . It contains AA Arg 31 - Gln 163
Predicted N-terminus: His
Request for sequence
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 16.8 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-17F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
获取HPLC数据
Immobilized Human IL-17F, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
Immobilized Human IL-17F, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag with a linear range of 0.016-0.25 μg/mL (Routinely tested).
Protocol
Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Human IL-17F, His Tag with an affinity constant of 219 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag on Protein A Biosensor, can bind Human IL-17F, His Tag with an affinity constant of 1.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-17F, His Tagon NTA Biosensor, can bind Human IL-17 RC, Fc Tagith an affinity constant of 100 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
