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Human OX40 Ligand / TNFSF4 Protein, Fc Tag (active trimer) (MALS verified)

Human OX40 Ligand / TNFSF4 Protein, Fc Tag (active trimer) (MALS verified)
别名: OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L
储存条件: -20℃ 
Human OX40 Ligand / TNFSF4 Protein, Fc Tag (active trimer) (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-OXL-H5266-100ug 100ug 现货 2900.00 2900
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熔点:
密度:
储存条件: -20℃

Human OX40 Ligand / TNFSF4 Protein, Fc Tag (active trimer) (MALS verified)

分子别名(Synonym)

OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L

表达区间及表达系统(Source)

Human OX40 Ligand, Fc Tagis expressed from human 293 cells. It contains AA Gln 51 - Leu 183

Predicted N-terminus: Pro

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the N-terminus.

The protein has a calculated MW of 74.0 kDa. The protein migrates as 130-210 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) SDS-PAGE gel

Human OX40 Ligand, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human OX40 Ligand, Fc Tag is more than 90% and the molecular weight of this protein is around 160-196 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human OX40 Protein, His Tag (MALS verified) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag  with a linear range of 0.1-1 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Cynomolgus / Rhesus macaque OX40, His Tag  at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag  with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Biotinylated Human OX40, Avitag,His Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human OX40 Ligand, Fc Tag with a linear range of 0.1-0.4 ng/mL (Routinely tested).

 

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